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mouse anti human trf2 primary antibody  (Novus Biologicals)


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    Novus Biologicals mouse anti human trf2 primary antibody
    A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and <t>TRF2</t> telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).
    Mouse Anti Human Trf2 Primary Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+trf2+primary+antibody/pmc03739759-58-6-12?v=Novus+Biologicals
    Average 90 stars, based on 4 article reviews
    mouse anti human trf2 primary antibody - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Human Umbilical Cord Matrix Stem Cells Maintain Multilineage Differentiation Abilities and Do Not Transform during Long-Term Culture"

    Article Title: Human Umbilical Cord Matrix Stem Cells Maintain Multilineage Differentiation Abilities and Do Not Transform during Long-Term Culture

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0071374

    A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and TRF2 telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).
    Figure Legend Snippet: A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and TRF2 telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).

    Techniques Used: Cell Culture, Activity Assay, Real-time Polymerase Chain Reaction, Positive Control, Southern Blot, Molecular Weight, Control, Marker



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    mouse anti human trf2 primary antibody  (Novus Biologicals)
    90
    Novus Biologicals mouse anti human trf2 primary antibody
    A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and <t>TRF2</t> telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).
    Mouse Anti Human Trf2 Primary Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+trf2+primary+antibody/pmc03739759-58-6-12?v=Novus+Biologicals
    Average 90 stars, based on 1 article reviews
    mouse anti human trf2 primary antibody - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    mouse monoclonal anti-human trf2 primary antibodies 4a794  (Millipore)
    90
    Millipore mouse monoclonal anti-human trf2 primary antibodies 4a794
    A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and <t>TRF2</t> telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).
    Mouse Monoclonal Anti Human Trf2 Primary Antibodies 4a794, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+trf2+primary+antibody/pmc03338753-215-11-18?v=Millipore
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti-human trf2 primary antibodies 4a794 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and TRF2 telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).

    Journal: PLoS ONE

    Article Title: Human Umbilical Cord Matrix Stem Cells Maintain Multilineage Differentiation Abilities and Do Not Transform during Long-Term Culture

    doi: 10.1371/journal.pone.0071374

    Figure Lengend Snippet: A. Representative normal G-banded karyotype of cultured UCMSC. B. Relative TRAP activity per microgram of protein lysate. Telomerase activity of UCMSC remained below the positive cut-off represented by heat inactivated (HI: 85°C for 10 min) HeLa cell extracts. C. Quantitative PCR confirmed the absence of hTERT gene transcripts in UCMSC at P9 and P18. HeLa cell cDNA was used as a positive control. D. Representative Southern blot analysis of telomere length. Lane 1 represents molecular weight markers. Telomere length of UCMSC shortened gradually between P3 and P18 (lane 2–4). Control HeLa cells (lane 5) displayed short telomeres, typical of most telomerase-positive cancer cell lines, while U2OS cells (lane 6) displayed the typical long and heterogeneous TRF pattern of ALT-positive cells. E. Cultured UCMSC display signs of telomere dysfunction induced cell senescence. Cells from P3, P9 and P18 were immunostained for 53BP1 DNA damage marker (red) and TRF2 telomeric protein (green). Co-localization of 53BP1 foci with TRF2 was scored on at least 50 nuclei to determine telomere dysfunction induced DNA damage foci (TIF). F. Quantification (%) of nuclei presenting 53BP1 foci at P3, P9 and P18. Percentage of nuclei presenting co-localization of 53BP1 with TRF2 in UCMSC at selected passages. (Scale bar 5 µm).

    Article Snippet: Fixed cells were incubated with a mouse anti-human TRF2 primary antibody (1/500; Imgenex, San Diego, USA) and with a rabbit anti-human 53BP1 antibody (1/250; Novus Biologicals, Cambridge, UK) overnight at 4°C.

    Techniques: Cell Culture, Activity Assay, Real-time Polymerase Chain Reaction, Positive Control, Southern Blot, Molecular Weight, Control, Marker